Development of an immobilized biocatalyst based on Bacillus psychrosaccharolyticus NDT for the preparative synthesis of trifluridine and decytabine
| dc.contributor.author | Fresco-Taboada, Alba | |
| dc.contributor.author | Serra, Immacolata | |
| dc.contributor.author | Arroyo Sánchez, Miguel | |
| dc.contributor.author | Fernández Lucas, Jesús | |
| dc.contributor.author | Mata Riesco, Isabel de la | |
| dc.contributor.author | Terreni, Marco | |
| dc.date.accessioned | 2016-01-07T07:53:36Z | |
| dc.date.available | 2016-01-07T07:53:36Z | |
| dc.date.issued | 2016 | |
| dc.description.abstract | The immobilization of Bacillus psychrosaccharolyticus nucleoside 2′-deoxyribosyltransferase was deeply investigated and finally optimized. The best immobilization procedure resulted to be ionic adsorption on PEI 600 Da agarose followed by crosslinking with 70% oxidized dextran (20 kDa). The percentage of recovered activity was further improved (from 21% to 33%) by the addition of 20% glycerol to the immobilization mixture. The resulting biocatalyst showed a stability profile similar to that of the soluble enzyme and it was used for the preparative synthesis of trifluridine and decytabine obtaining conversions ranging from 50% to 76%. | spa |
| dc.description.filiation | UEM | spa |
| dc.description.impact | 4.636 JCR (2016) Q1, 6/72 Chemistry, Applied, 30/146 Chemistry, Physical, 12/135 Engineering, Chemical | spa |
| dc.description.sponsorship | Comunidad Autónoma de Madrid (Spain) (Grant S2009/PPQ-1752) and Spanish Ministry of Science and Innovation (CTQ2009-11543) | spa |
| dc.identifier.citation | Fresco-Taboada, A., Serra, I., Arroyo, M., Fernández-Lucas, J., de la Mata, I., & Terreni, M. (2016). Development of an immobilized biocatalyst based on Bacillus psychrosaccharolyticus NDT for the preparative synthesis of trifluridine and decytabine. Catalysis Today, 259, 197-204. | spa |
| dc.identifier.doi | 10.1016/j.cattod.2015.06.032 | |
| dc.identifier.issn | 09205861 | |
| dc.identifier.uri | http://hdl.handle.net/11268/4768 | |
| dc.language.iso | eng | spa |
| dc.peerreviewed | Si | spa |
| dc.relation.projectID | Grant S2009/PPQ-1752 | spa |
| dc.rights.accessRights | restricted access | spa |
| dc.subject.uem | Biotecnología | spa |
| dc.subject.unesco | Biotecnología | spa |
| dc.title | Development of an immobilized biocatalyst based on Bacillus psychrosaccharolyticus NDT for the preparative synthesis of trifluridine and decytabine | spa |
| dc.type | journal article | spa |
| dspace.entity.type | Publication | |
| relation.isAuthorOfPublication | 65bdb4fa-7adf-42ce-b40e-421a62e05239 | |
| relation.isAuthorOfPublication.latestForDiscovery | 65bdb4fa-7adf-42ce-b40e-421a62e05239 |