A Bump-Hole Strategy for Increased Stringency of Cell-Specific Metabolic Labeling of RNA

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dc.contributor.authorNguyen, Kim
dc.contributor.authorKubota, Miles
dc.contributor.authorArco Arrieta, Jon del
dc.contributor.authorFeng, Chao
dc.contributor.authorSingha, Monika
dc.contributor.authorBeasley, Samantha
dc.contributor.authorSakr, Jasmine
dc.contributor.authorGandhi, Sunil P.
dc.contributor.authorBlurton-Jones, Matthew
dc.contributor.authorSpitale, Robert C.
dc.contributor.authorEt al.
dc.date.accessioned2022-03-27T11:08:26Z
dc.date.available2022-03-27T11:08:26Z
dc.date.issued2020
dc.description.abstractProfiling RNA expression in a cell-specific manner continues to be a grand challenge in biochemical research. Bioorthogonal nucleosides can be utilized to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Herein, we design and demonstrate that uracil phosphoribosyltransferase can be engineered to match 5-vinyluracil for cell-specific metabolic labeling of RNA with exceptional specificity and stringency.spa
dc.description.filiationUEMspa
dc.description.impact5.100 JCR (2020) Q2, 87/295 Biochemistry & Molecular Biologyspa
dc.description.impact1.899 SJR (2020) Q2, 62/438 Biochemistryspa
dc.description.impactNo data IDR 2020spa
dc.description.sponsorshipUniversity of California Systemspa
dc.description.sponsorshipUnited States Department of Health & Human Services National Institutes of Health (NIH) - USA (V1DP2GM119164)spa
dc.description.sponsorshipNIH Director's New Innovator Award (5T32CA009054-40)spa
dc.identifier.citationNguyen, K., Kubota, M., Arco, J., Feng, C., Singha, M., Beasley, S., Sakr, J., Gandhi, S. P., Blurton-Jones, M., Fernández Lucas, J., & Spitale, R. C. (2020). A Bump-Hole Strategy for Increased Stringency of Cell-Specific Metabolic Labeling of RNA. ACS Chemical Biology, 15(12), 3099–3105. https://doi.org/10.1021/acschembio.0c00755spa
dc.identifier.doi10.1021/acschembio.0c00755
dc.identifier.issn1554-8929
dc.identifier.issn1554-8937
dc.identifier.urihttp://hdl.handle.net/11268/10954
dc.language.isoengspa
dc.peerreviewedSispa
dc.relation.publisherversionhttps://doi.org/10.1021/acschembio.0c00755spa
dc.rights.accessRightsopen accessspa
dc.subject.otherNucleósidosspa
dc.subject.unescoBioquímicaspa
dc.subject.unescoBiología celularspa
dc.titleA Bump-Hole Strategy for Increased Stringency of Cell-Specific Metabolic Labeling of RNAspa
dc.typejournal articlespa
dspace.entity.typePublication
relation.isAuthorOfPublication22b24c8d-eef2-4ba1-aa37-57f350485e51
relation.isAuthorOfPublication.latestForDiscovery22b24c8d-eef2-4ba1-aa37-57f350485e51

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