Kinetics of Protein Sα-Klotho after an eccentric exercise in young people with good physical condition

Abstract

INTRODUCTION: High levels of soluble fraction of alpha Klotho (sαKl) have been related to longevity, with a decrease in degenerative processes and with an anti-inflammatory effect on the organism. On the other hand, an increase in sαKl levels has been described immediately after having completed an acute aerobic exercise1. The literature suggests that eccentric exercise would not have the same acute effect on this protein2 but does not report the behavior of the protein during the recovery period. Taking this background, the main objective was to analyze the acute effect of an eccentric exercise on the sαKl protein and its kinetics during the 72 hours following it. METHODS: Participation of 15 male subjects from 18 to 35 years old with good physical condition (VO2max. 50 and 70ml/kg/min). All of them underwent an acute exercise session consisting of 100 jumps from a 50cm drawer followed by a plyometric phase, the entire execution with 10% of their additional body weight. Physical condition (VO2max) was measured with ergo spirometry and body composition (BAI: percentage of fat; ASM: Muscular Index Appen MM/height2 [kg/m2]; VATarea: visceral fat) by dual X-ray densitometry (DEXA), they measured for the sample recruitment. As a primary variable the serum levels of sαKL measured by ELISA (αKlotho Assay kit, IBL) in T1 (acute pre-intervention), T2 (acute post-intervention), T3 (24 hours after acute exercise), T4 (48 hours later) and T5 (72 hours later). RESULTS: Homogeneous sample with VO2max averages of 57.4 ± 5.2ml/kg/min; BAI 17.7 ± 2.7%; VATarea 47.21 ± 11.6cm2 and ASM 8.3 ± 0.6. The average for the concentrations of sαKl at times T1, T2, T3, T4 and T5 are 1064.05 ± 211.23pg/ml; 982.25 ± 226.65pg/ml; 1464.73 ± 262.12pg/ml; 1465.97 ± 321.60pg/ml and 1309.68 ± 595.45pg/ml, respectively. They only present significant differences between subjects in T5 (p=0.018). Between the assessment moments the sαKl levels are statistically significant between T1 and T2 (p=0.003) and between T2 and T3 (p=0.001). There is a significant decrease between the acute pre and post-session moments2, however, this difference may not be relevant from a biological point of view. The main increase is observed between the post-intervention time and the following 24 hours, maintaining, in all cases, these levels between 24 and 48 hours, and finally reaching baseline values in 40% of the subjects at 72 hours. CONCLUSION: According to the literature the results suggest that an eccentric exercise does not have immediate acute effect on soluble alpha Klotho2, but the behavior of 24, 48 and 72 hours could be an inflammatory response to this type of exercise.