Comparative Micro-CT Analysis of Minimally Invasive Endodontic Systems Using 3D-Printed Replicas and Natural Teeth

Abstract

Background: This study aimed to compare the shaping abilities of modern minimally invasive endodontic systems using natural teeth and 3D-printed resin replicas. These replicas offer a standardized approach for studying root canal preparation while eliminating the variability and scarcity of natural teeth. (2) Methods: Eleven mandibular molars with Vertucci class IV anatomy and their 3D-printed replicas (n = 132 canals) were scanned using micro-CT before and after preparation with six rotary systems. Shaping abilities were assessed by comparing volume, surface area, and unprepared areas between natural teeth and their 3D replicas, focusing on the apical third. Statistical analysis included the Shapiro–Wilk test to assess data normality and ANOVA and t-tests to compare different endodontic systems. (3) Results: Both qualitative and quantitative analyses revealed high similarity between natural teeth and 3D replicas. No significant differences in volume or surface area were found except in the apical third, where 3D replicas showed slightly larger increases in volume. (4) Conclusions: 3D resin replicas closely mimic natural teeth and provide a practical tool for assessing the shaping abilities of endodontic systems. This study demonstrates that 3D-printed models are suitable for endodontic research, offering a standardized and accessible alternative to natural teeth.