Resumen:
We investigated the cytoprotective role of the dietary polyphenols on putative damage
induced by Amadori adducts in Human Peritoneal Mesothelial Cells (HPMCs). Increased
accumulation of early products of non-enzymatic protein glycation—Amadori adducts—in the
peritoneal dialysis fluid due to their high glucose, induces severe damage in mesothelial cells
during peritoneal dialysis. Dietary polyphenols reportedly have numerous health benefits in
various diseases and have been used as an efficient antioxidant in the context of several oxidative
stress-related pathologies. HPMCs isolated from different patients were exposed to Amadori
adducts (highly glycated haemoglobin, at physiological concentrations), and subsequently treated
with several polyphenols, mostly presented in our Mediterranean diet. We studied several
Amadori-induced effects in pro-apoptotic and oxidative stress markers, as well as the expression of
several pro-inflammatory genes (nuclear factor-kappaB, NF-kB; inducible Nitric Oxide synthetase,
iNOS), different caspase-activities, level of P53 protein or production of different reactive oxygen
species in the presence of different polyphenols. In fact, cytoprotective agents ...