Muscle molecular adaptations to endurance exercise training are conditioned by glycogen availability: A proteomics‐based analysis in the McArdle mouse model
Muscle molecular adaptations to endurance exercise training are conditioned by glycogen availability: A proteomics‐based analysis in the McArdle mouse model
Fiuza Luces, María del Carmen; Santos-Lozano, Alejandro; Llavero Bernal, Francisco; Campo, Rocío; Nogales-Gadea, Gisela; Díez Bermejo, Jorge; Baladrón, Carlos; González-Murillo, África; Arenas, Joaquín; Lucía Mulas, Alejandro; Et al.
McArdle's disease is an inborn disorder of skeletal muscle glycogen metabolism that results in blockade of glycogen breakdown due to mutations in the myophosphorylase gene. We recently developed a mouse model carrying the homozygous p.R50X common human mutation (McArdle mouse), facilitating the study of how glycogen availability affects muscle molecular adaptations to endurance exercise training. Using quantitative differential analysis by liquid chromatography with tandem mass spectrometry, we analysed the quadriceps muscle proteome of 16‐week‐old McArdle (n = 5) and wild‐type (WT) (n = 4) mice previously subjected to 8 weeks’ moderate‐intensity treadmill training or to an equivalent control (no training) period. Protein networks enriched within the differentially expressed proteins with training in WT and McArdle mice were assessed by hypergeometric enrichment analysis. Whereas endurance exercise training improved the estimated maximal aerobic capacity of both WT and McArdle mice as compared with controls, it was ∼50% lower than normal in McArdle mice before and after training. We found a remarkable difference in the protein networks involved in muscle tissue adaptations induced by endurance ex...