Resumen:
The subiculum, which has a strategic position in controlling hippocampal activity, is receiving significant attention in epilepsy research.
However, the functional organization of subicular circuits remains unknown. Here, we combined different recording and analytical
methods to study focal and widespread population activity in the isolated subiculum in zeroMg2 media. Patch and field recordings were
combined to examine the contribution of different cell types to population activity. The properties of cells leading field activity were
examined. Predictive factors for a cell to behave as leader included exhibiting the bursting phenotype, displaying a low firing threshold,
and having more distal apical dendrites. A subset of bursting cells constituted the first glutamatergic type that led a recruitment process
that subsequently activated additional excitatory as well as inhibitory cells. This defined a sequence of synaptic excitation and inhibition
that was studied by measuring the associated conductance changes and the evolution of the composite reversal potential. It is shown that
inhibition was time-locked to excitation, which shunted excitatory inputs and suppressed firing during focal a...