Resumen:
Statement of problem: New vital pulp therapy concept, using GSK 3 inhibitors (Tideglusib) incorporated in resorbable scaffold, is being introduced nowadays into literature promising superior results than the present pulp caping materials especially in inducing full dentin regeneration and the induction of tooth regeneration. Using this newly introduced drug is still under investigation for its safe in-vivo use and the confirmation of the expected outcomes.
Aims of the study: The current study aims at assessing the regenerative the two proposed GSK3 inhibitors drugs namely (Tideglusib & CHIR99021) on activation of Wnt/beta catenin pathway regarding the proliferative capacity odontogenic differentiation potential of human dental pulp stem cells.
Materials and Methods: Study design set for this study is in-vitro study. Human Dental Pulp Stem Cells (hDPSC's) was obtained from extracted sound anterior teeth for periodontal and aesthetic problems, after receiving their informed consents and was isolated and cultivated according to Gronthos et al., 2000 technique. Flow cytometric analysis was used to confirm the mesenchymal stem cells properties of the hDPSCs. Wnt Induction Assay was achieved by...