Sánchez-Arrones, LuisaNieto-Lopez, FranciscoSánchez-Camacho Blázquez, CristinaCarreres, M. IsabelHerrera, EloisaOkada, AmiBovolenta, Paola2013-11-272013-11-272013Sánchez-Arrones, L., Nieto-López, F., Sánchez-Camacho, C., Carreres, M. I., Herrera, E., Okada, A., & Bovolenta, P. (2013). Shh/Boc signaling is required for sustained generation of ipsilateral projecting ganglion cells in the mouse retina. The Journal of Neuroscience, 33(20), 8596-8607.http://hdl.handle.net/11268/993Sonic Hedgehog (Shh) signaling is an important determinant of vertebrate retinal ganglion cell (RGC) development. In mice, there are two major RGC populations: (1) the Islet2-expressing contralateral projecting (c)RGCs, which both produce and respond to Shh; and (2) the Zic2-expressing ipsilateral projecting RGCs (iRGCs), which lack Shh expression. In contrast to cRGCs, iRGCs, which are generated in the ventrotemporal crescent (VTC) of the retina, specifically express Boc, a cell adhesion molecule that acts as a high-affinity receptor for Shh. In Boc −/− mutant mice, the ipsilateral projection is significantly decreased. Here, we demonstrate that this phenotype results, at least in part, from the misspecification of a proportion of iRGCs. In Boc−/− VTC, the number of Zic2-positive RGCs is reduced, whereas more Islet2/Shh-positive RGCs are observed, a phenotype also detected in Zic2 and Foxd1 null embryos.engjournal article10.1523/JNEUROSCI.2083-12.2013restricted accessOftalmologíaGenética humana