Abreu Silveira, ErickMoreno Pérez, SoniaBasso, AlessandraSerban, SimonaPestana Mamede, RitaTardioli, Paulo W.Sánchez Farinas, CristianeRocha Martín, JavierFernández Lorente, GloriaGuisan, José M.2018-01-022018-01-022017Silveira, E. A., Moreno-Perez, S., Basso, A., Serban, S., Mamede, R. P., Tardioli, P. W., ... & Guisan, J. M. (2017). Modulation of the regioselectivity of Thermomyces lanuginosus lipase via biocatalyst engineering for the Ethanolysis of oil in fully anhydrous medium. BMC Biotechnology, 17, 1-13. DOI: 10.1186/s12896-017-0407-91472-6750http://hdl.handle.net/11268/6935Enzymatic ethanolysis of oils (for example, high oleic sunflower oil containing 90% of oleic acid) may yield two different reaction products depending on the regioselectivity of the immobilized lipase biocatalyst. Some lipase biocatalysts exhibit a 1,3-regioselectivity and they produced 2 mols of fatty acid ethyl ester plus 1 mol of sn2-monoacylglycerol (2-MAG) per mol of triglyceride without the release of glycerol. Other lipase biocatalysts are completely non-regioselective releasing 3 mols of fatty acid ethyl ester and 1 mol of glycerol per mol of triglyceride. Lipase from Thermomyces lanuginosus (TLL) adsorbed on hydrophobic supports is a very interesting biocatalyst for the ethanolysis of oil. Modulation of TLL regioselectivity in anhydrous medium was intended via two strategies of TLL immobilization: a. - interfacial adsorption on different hydrophobic supports and b.- interfacial adsorption on a given hydrophobic support under different experimental conditions.engjournal article10.1186/s12896-017-0407-9open accessBiodieselEnzimaEnergía de la biomasa