Delgado, AmaiaCastagnola, RaffaellaGrande, Nicola MaríaLoroño Goikoechea, GaizkaConde Villar, Antonio JesúsEstévez Luaña, RobertoMarigo, LucaPérez Alfayate, Ruth2025-02-152025-02-152024Delgado, A., Castagnola, R., Grande, N. M., Loroño, G., Conde, A., Estevez, R., Marigo, L., & Pérez-Alfayate, R. (2024). A comparison of two fluorescent stains for the evaluation of bioceramic sealer penetration into the tubular dentine using confocal microscopy. Applied Sciences, 14(17), 7738. https://doi.org/10.3390/app141777382076-3417http://hdl.handle.net/11268/13675This study aimed to compare the percentage of bioceramic sealer penetration and maximum penetration sealer depending on the fluorescent staining method when evaluated with confocal laser scanning microscopy (CLSM). In total, 42 single-rooted premolars were instrumented. Two groups of 20 teeth each were filled using the single cone technique. TotalFill BC Sealer® mixed with Rhodamine B was used for the first group and TotalFill BC Sealer® mixed with Fluo-3 for the second group. Two teeth made up the control group. All teeth were sectioned at 3 and 6 mm from the apex and were analyzed with CLSM. The canal perimeter where the tubular penetration happened and the maximum penetration of each sample were calculated. There were no significant differences between the two groups in either the apical or medial sections. In terms of maximum penetration, Rhodamine B had greater tubular penetration in the apical sections, and it had similar results to Fluo-3 in the medial sections. TotalFill BC Sealer® has a higher tubular penetration in the medial slices than in the apical slices regardless of the fluorescent stain used. Rhodamine B has higher results than Fluo-3 in terms of the percentage of sealer penetration and maximum penetration.engAttribution-NonCommercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/Microscopía ConfocalMateriales de Obturación del Conducto Radicularjournal article10.3390/app14177738open accessOdontologíaTecnología médicaGoal 3: Ensure healthy lives and promote well-being for all at all ages