Acosta Bueno, JavierArco Arrieta, Jon delMartínez Pascual, SaraClemente Suárez, Vicente JavierFernández Lucas, Jesús2018-11-242018-11-242018Acosta, J., Arco, J. D., Martinez-Pascual, S., Clemente-Suárez, V. J., & Fernández-Lucas, J. (2018). One-pot multi-enzymatic production of purine derivatives with application in pharmaceutical and food industry. Catalysts, 8(1), 9. https://doi.org/10.3390/catal80100092073-4344http://hdl.handle.net/11268/7605Biocatalysis reproduce nature’s synthetic strategies in order to synthesize different organic compounds. Natural metabolic pathways usually involve complex networks to support cellular growth and survival. In this regard, multi-enzymatic systems are valuable tools for the production of a wide variety of organic compounds. Methods: The production of different purine nucleosides and nucleoside-50 -monophosphates has been performed for first time, catalyzed by the sequential action of 2 0 -deoxyribosyltransferase from Lactobacillus delbrueckii (LdNDT) and hypoxanthine-guanine-xanthine phosphoribosyltransferase from Thermus themophilus HB8 (TtHGXPRT). Results: The biochemical characterization of LdNDT reveals that the enzyme is active and stable in a broad range of pH, temperature, and ionic strength. Substrate specificity studies showed a high promiscuity in the recognition of purine analogues. Finally, the enzymatic production of different purine derivatives was performed to evaluate the efficiency of multi-enzymatic system LdNDT/TtHGXPRT. Conclusions: The production of different therapeutic purine nucleosides was efficiently catalyzed by LdNDT/TtHGXPRT. In addition, the resulting by-products were converted to IMP and GMP. Taking all of these features, this bioprocess entails an efficient, sustainable, and economical alternative to chemical synthetic methods.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal article10.3390/catal8010009open access