Arco Arrieta, Jon delSánchez Murcia, Pedro AlejandroMancheño, José MiguelGago, FedericoFernández Lucas, Jesús2019-10-142019-10-142018Arco, J., Sánchez, P. A., Mancheño, J. M., Gago, F., & Fernández, J. (2018). Characterization of an atypical, thermostable, organic solvent-and acid-tolerant 2′-deoxyribosyltransferase from Chroococcidiopsis thermalis. Applied Microbiology and Biotechnology, 102(16), 6947-6957. https://doi.org/10.1007/s00253-018-9134-y0175-75981432-0614http://hdl.handle.net/11268/8352In our search for thermophilic and acid-tolerant nucleoside 2′-deoxyribosyltransferases (NDTs), we found a good candidate in an enzyme encoded by Chroococcidiopsis thermalis PCC 7203 (CtNDT). Biophysical and biochemical characterization revealed CtNDT as a homotetramer endowed with good activity and stability at both high temperatures (50–100 °C) and a wide range of pH values (from 3 to 7). CtNDT recognizes purine bases and their corresponding 2′-deoxynucleosides but is also proficient using cytosine and 2′-deoxycytidine as substrates. These unusual features preclude the strict classification of CtNDT as either a type I or a type II NDT and further suggest that this simple subdivision may need to be updated in the future. Our findings also hint at a possible link between oligomeric state and NDT’s substrate specificity. Interestingly from a practical perspective, CtNDT displays high activity (80–100%) in the presence of several water-miscible co-solvents in a proportion of up to 20% and was successfully employed in the enzymatic production of several therapeutic nucleosides such as didanosine, vidarabine, and cytarabine.engjournal article10.1007/s00253-018-9134-yrestricted accessBiología molecularEnzimasBiología molecularEnzima