Nguyen, KimKubota, MilesArco Arrieta, Jon delFeng, ChaoSingha, MonikaBeasley, SamanthaSakr, JasmineGandhi, Sunil P.Blurton-Jones, MatthewSpitale, Robert C.Et al.2022-03-272022-03-272020Nguyen, K., Kubota, M., Arco, J., Feng, C., Singha, M., Beasley, S., Sakr, J., Gandhi, S. P., Blurton-Jones, M., Fernández Lucas, J., & Spitale, R. C. (2020). A Bump-Hole Strategy for Increased Stringency of Cell-Specific Metabolic Labeling of RNA. ACS Chemical Biology, 15(12), 3099–3105. https://doi.org/10.1021/acschembio.0c007551554-89291554-8937http://hdl.handle.net/11268/10954Profiling RNA expression in a cell-specific manner continues to be a grand challenge in biochemical research. Bioorthogonal nucleosides can be utilized to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Herein, we design and demonstrate that uracil phosphoribosyltransferase can be engineered to match 5-vinyluracil for cell-specific metabolic labeling of RNA with exceptional specificity and stringency.engNucleósidosjournal article10.1021/acschembio.0c00755open accessBioquímicaBiología celular