Santos Lozano, AlejandroValenzuela Ruiz, Pedro LuisLlavero Bernal, FranciscoLista, SimoneCarrera Bastos, PedroHampel, HaraldPareja Galeano, HeliosGonzález Gálvez, BeatrizLópez, Juan AntonioLucía Mulas, AlejandroEt al.2020-03-272020-03-272020Santos-Lozano, A., Valenzuela Ruiz, P. L., Llavero, F., Lista, S., Carrera-Bastos, P., Hampel, H., Pareja-Galeano, H., González Gálvez, B., López, J. A., Vázquez, J., Emanuele, E., Zugaza, J. L., & Lucía Mulas, A. (2020). Successful aging: Insights from proteome analyses of healthy centenarians. Aging, 12(4), 3502–3515. https://doi.org/10.18632/aging.1028261945-4589http://hdl.handle.net/11268/8860Healthy aging depends on a complex gene-environment network that is ultimately reflected in the expression of different proteins. We aimed to perform a comparative analysis of the plasma proteome of healthy centenarians (n=9, 5 women, age range 100–103 years) with a notably preserved ambulatory capacity (as a paradigm of ‘successful’ aging), and control individuals who died from a major age-related disease before the expected life expectancy (n=9, 5 women, age range: 67–81 years), and while having impaired ambulatory capacity (as a paradigm of ‘unsuccessful’ aging). We found that the expression of 49 proteins and 86 pathways differed between the two groups. Overall, healthy centenarians presented with distinct expression of proteins/pathways that reflect a healthy immune function, including a lower pro-inflammatory status (less ‘inflammaging’ and autoimmunity) and a preserved humoral immune response (increased B cell-mediated immune response). Compared with controls, healthy centenarians also presented with a higher expression of proteins involved in angiogenesis and related to enhanced intercellular junctions, as well as a lower expression of proteins involved in cardiovascular abnormalities. The identification of these proteins/pathways might provide new insights into the biological mechanisms underlying the paradigm of healthy aging.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal article10.18632/aging.102826open accessAncianosCalidad de vidaGenética humanaAncianoCalidad de vidaGenética humana