Rinaldi, FrancescaFernández Lucas, JesúsFuente, Diego de laZheng, ChangpingBavaro, TeodoraPeters, BenjaminMassolini, GabriellaAnnunziata, FrancescaConti, PaolaCalleri, EnricaEt al.2022-03-242022-03-242020Rinaldi, F., Fernández-Lucas, J., Fuente, D., Zheng, C., Bavaro, T., Peters, B., Massolini, G., Annunziata, F., Conti, P., Mata, I., Terreni, M., & Calleri, E. (2020). Immobilized enzyme reactors based on nucleoside phosphorylases and 2′-deoxyribosyltransferase for the in-flow synthesis of pharmaceutically relevant nucleoside analogues. Bioresource Technology, 307, 123258. https://doi.org/10.1016/j.biortech.2020.1232580960-85241873-2976http://hdl.handle.net/11268/10936In this work, a mono- and a bi-enzymatic analytical immobilized enzyme reactors (IMERs) were developed as prototypes for biosynthetic purposes and their performances in the in-flow synthesis of nucleoside analogues of pharmaceutical interest were evaluated. Two biocatalytic routes based on nucleoside 2′-deoxyribosyltransferase from Lactobacillus reuteri (LrNDT) and uridine phosphorylase from Clostridium perfrigens (CpUP)/purine nucleoside phosphorylase from Aeromonas hydrophila (AhPNP) were investigated in the synthesis of 2′-deoxy, 2′,3′-dideoxy and arabinonucleoside derivatives. LrNDT-IMER catalyzed the synthesis of 5-fluoro-2′-deoxyuridine and 5-iodo-2′-deoxyuridine in 65–59% conversion yield, while CpUP/AhPNP-IMER provided the best results for the preparation of arabinosyladenine (60% conversion yield). Both IMERs proved to be promising alternatives to chemical routes for the synthesis of nucleoside analogues. The developed in-flow system represents a powerful tool for the fast production on analytical scale of nucleosides for preliminary biological tests.engReactores biológicosNucleósidosjournal article10.1016/j.biortech.2020.123258restricted accessBiología molecular