Abreu Silveira, ErickMoreno Pérez, SoniaBasso, AlessandraSerban, SimonaPestana Mamede, RitaTardioli, Paulo W.Sánchez Farinas, CristianeCastejón, NataliaFernández Lorente, GloriaRocha Martín, JavierGuisan, José M.2022-02-202022-02-202019Abreu Silveira, E., Moreno-Pérez, S., Basso, A., Serban, S., Pestana-Mamede, R., Tardioli, P. W., Fariñas, C. S., Castejón, N., Ferández, G., Rocha, J., & Guisan, J. M. (2019). Biocatalyst engineering of Thermomyces Lanuginosus lipase adsorbed on hydrophobic supports: Modulation of enzyme properties for ethanolysis of oil in solvent-free systems. Journal of Biotechnology, 289, 126–134. https://doi.org/10.1016/j.jbiotec.2018.11.0140168-16561873-4863http://hdl.handle.net/11268/10774Different immobilized biocatalysts of Thermomyces lanuginosus lipase (TLL) exhibited different properties for the ethanolysis of high oleic sunflower oil in solvent-free systems. TLL immobilized by interfacial adsorption on octadecyl (C-18) supports lost its 1,3-regioselectivity and produced more than 99% of ethyl esters. This reaction was influenced by mass-transfer limitations. TLL adsorbed on macroporous C-18 supports (616 Å of pore diameter) was 10-fold more active than TLL adsorbed on mesoporous supports (100–200 Å of pore diameter) in solvent-free systems. Both derivatives exhibited similar activity when working in hexane in the absence of diffusional limitations. In addition, TLL adsorbed on macroporous Purolite C-18 was 5-fold more stable than TLL adsorbed on mesoporous Sepabeads C-18. The stability of the best biocatalyst was 20-fold lower in anhydrous oil than in anhydrous hexane. Mild PEGylation of immobilized TLL greatly increased its stability in anhydrous hexane at 40 °C, fully preserving the activity after 20 days. In anhydrous oil at 40 °C, PEGylated TLL-Purolite C-18 retained 65% of its initial activity after six days compared to 10% of the activity retained by the unmodified biocatalyst. Macroporous and highly hydrophobic supports (e.g., Purolite C-18) seem to be very useful to prepare optimal immobilized biocatalysts for ethanolysis of oils by TLL in solvent-free systems.engBiotecnologíaLipasaBiocatálisisjournal article10.1016/j.jbiotec.2018.11.014restricted accessBioquímicaEnzima