Rodríguez Sánchez, ElenaNavarro García, José AlbertoAceves Ripoll, JenniferAbarca Zabalía, JudithSusmozas Sánchez, AndreaBada Bosch, TeresaHernández Martínez, EduardoMérida Herrero, EvangelinaRuilope Urioste, Luis MiguelRuiz Hurtado, GemaEt al.2020-06-202020-06-202020Rodríguez-Sánchez, E., Navarro-García, J. A., Aceves-Ripoll, J., Abarca-Zabalía, J., Susmozas-Sánchez, A., Bada-Bosch, T., Hernández Martínez, E., Mérida-Herrero, E., Andrés, A., Praga, M., Fernández-Ruiz, M., Aguado, J. M., Segura, J., Ruilope Urioste, L. M., & Ruiz-Hurtado, G. (2020). Variations in Circulating Active MMP-9 Levels during Renal Replacement Therapy. Biomolecules, 10(4), 505. https://doi.org/10.3390/biom100405052218-273Xhttp://hdl.handle.net/11268/8985Renal replacement therapy (RRT) is complicated by a chronic state of inflammation and a high mortality risk. However, different RRT modalities can have a selective impact on markers of inflammation and oxidative stress. We evaluated the levels of active matrix metalloproteinase (MMP)-9 in patients undergoing two types of dialysis (high-flux dialysis (HFD) and on-line hemodiafiltration (OL-HDF)) and in kidney transplantation (KT) recipients. Active MMP-9 was measured by zymography and ELISA before (pre-) and after (post-) one dialysis session, and at baseline and follow-up (7 and 14 days, and 1, 3, 6, and 12 months) after KT. Active MMP-9 decreased post-dialysis only in HFD patients, while the levels in OL-HDF patients were already lower before dialysis. Active MMP-9 increased at 7 and 14 days post-KT and was restored to baseline levels three months post-KT, coinciding with an improvement in renal function and plasma creatinine. Active MMP-9 correlated with pulse pressure as an indicator of arterial stiffness both in dialysis patients and KT recipients. In conclusion, active MMP-9 is better controlled in OL-HDF than in HFD and is restored to baseline levels along with stabilization of renal parameters after KT. Active MMP-9 might act as a biomarker of arterial stiffness in RRT.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal article10.3390/biom10040505open accessDiálisisTecnología médicaEnfermedad cardiovascularTratamiento médicoTecnología médica