Romero Fernández, MaríaMoreno Pérez, SoniaOrrego, Alejandro H.Martins de Oliveira, SandroSantamaría, Ramón I.Díaz, MargaritaGuisan, José M.Rocha Martín, Javier2019-07-122019-07-122018Romero-Fernández, M., Moreno-Perez, S., H. Orrego, A., de Oliveira, S., I. Santamaría, R., Díaz, M., … & Rocha-Martin, J. (2018). Designing continuous flow reaction of xylan hydrolysis for xylooligosaccharides production in packed-bed reactors using xylanase immobilized on methacrylic polymer-based supports. Bioresource Technology, 266, 249–258. https://doi.org/10.1016/j.biortech.2018.06.0700960-85241873-2976http://hdl.handle.net/11268/8127The present study focuses on the development and optimization of a packed-bed reactor (PBR) for continuous production of xylooligosaccharides (XOS) from xylan. For this purpose, three different methacrylic polymer-based supports (Relizyme R403/S, Purolite P8204F and Purolite P8215F) activated with glyoxyl groups were morphologically characterized and screened for the multipoint covalent immobilization of a xylanase. Based on its physical and mechanical properties, maximum protein loading and thermal stability, Relizyme R403/S was selected to set up a PRB for continuous production of XOS from corncob xylan. The specific productivity for XOS at 10 mL/min flow rate was 3277 gXOS genzyme−1 h−1 with a PBR. This PBR conserved >90% of its initial activity after 120 h of continuous operation.engjournal article10.1016/j.biortech.2018.06.070restricted accessEnzimasBiología molecularBiología molecularEnzima