Ramírez de Molina, AnaGallego-Ortega, DavidSarmentero, JacintoBáñez-Coronel, MónicaMartín Cantalejo, YolandaLacal, Juan Carlos2016-08-032016-08-032005Ramírez de Molina, A., Gallego-Ortega, D., Sarmentero, J., Bañez-Coronel, M., Martín-Cantalejo, Y., & Lacal, J. C. (2005). Choline kinase is a novel oncogene that potentiates RhoA-induced carcinogenesis. Cancer Research, 65(13), 5647-5653.0008547215387445http://hdl.handle.net/11268/5533Choline kinase is overexpressed in human breast, lung, colorectal, and prostate tumors, a finding that suggests the involvement of this enzyme in carcinogenesis. Here we show that overexpression of choline kinase induce oncogenic transformation of human embryo kidney fibroblasts and canine epithelial Madin-Darby canine kidney cells. Choline kinase lays downstream of RhoA signaling and is activated through ROCK kinase, one of the best-characterized RhoA effectors. In keeping with this, coexpression of RhoA and choline kinase potentiates both anchorage independent growth and tumorigenesis. Finally, choline kinase–mediated transformation is sensitive to MN58b, a well-characterized specific choline kinase inhibitor. These results provide the definitive evidence that choline kinase has oncogenic properties and that choline kinase inhibition constitutes a novel valid antitumor strategy.engjournal article10.1158/0008-5472.CAN-04-4416open accessOncologíaCáncer