Alejandro Matero, SandraAzami-Conesa, IrisMartín-Maldonado Jiménez, BárbaraPastor Tiburón, NataliaMartín-Hernández, RaquelGonzález González, FernandoGómez Muñoz, Teresa2023-02-152023-02-152022Alejandro Mateo, S., Azami-Conesa, I., Martín-Maldonado, B., Pastor-Tiburón, N., Martín-Hernández, R., González-González, F., & Gómez-Muñoz, M. T. (2022). Adaptation of the classical end-point ITS-PCR for the diagnosis of avian trichomonosis to a real-time PCR reveals Bonelli’s eagle as a new host for Trichomonas gypaetinii. Parasitology Research, 121(12), 3663-3670. https://doi.org/10.1007/s00436-022-07693-30932-01131432-1955http://hdl.handle.net/11268/11785Avian trichomonosis is a parasitic disease caused mainly by Trichomonas gallinae and other Trichomonas species. It can be asymptomatic, or it can produce a necrotic lesion in the upper digestive tract and spread to other organs, causing the death of the infected birds. In this study, we aimed to evaluate an adapted real-time PCR method for the diagnosis of different genotypes and species of avian oropharyngeal trichomonads. Fifty-six samples from the oropharynx of Bonelli’s eagles (Aquila fasciata) obtained between 2018 and 2019 were analyzed using the real-time PCR and the end-point PCR, both targeting trichomonads ITS, and the results were compared by a coefficient of agreement. All positive samples were sequenced. The analysis showed a higher percentage of detection of real-time PCR ITS compared with end-point PCR ITS (64.3 vs 55.4%), and good agreement value (Kappa = 0.816). Melting temperature value for resulting amplicons of real-time PCR for avian trichomonads was 83.45 ± 0.72 °C. Genotypes A, D, and III were found among the sequences. Moreover, Trichomonas gypaetinii, a common species in scavenger birds, is reported for the first time in Bonelli’s eagles.engAttribution 4.0 International (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/journal article10.1007/S00436-022-07693-3open accessVeterinariaAveParasitología