Lucas Moreno, Beatriz deBernal, AuroraPérez, Laura M.San Martín, NuriaGonzález Gálvez, Beatriz2016-05-092016-05-092016de Lucas, B., Bernal, A., Pérez, L. M., San Martín, N., & Gálvez, B. G. (2016). Membrane Blebbing Is Required for Mesenchymal Precursor Migration. PloS one, 11(3), e0150004.19326203http://hdl.handle.net/11268/5120Mesenchymal precursors (MPs) present some advantageous features, such as differentiation and migration, which make them promising candidates for cell therapy. A better understanding of MP migration characteristics would aid the development of cell delivery protocols. Traditionally, cell migration is thought to occur only through the formation of lamellipodia. More recently, contractility-driven bleb formation has emerged as an alternative mechanism of motility. Here we report that MPs derived from different tissues present spontaneously dynamic cytoplasmic projections in sub-confluent culture, which appear as a combination of lamellipodia with blebs in the leading edge. Upon initial seeding, however, only bleb structures could be observed. Immunofluorescence revealed the presence of pERM, RhoA and F-actin during the blebbing process. Results from migration assays in the presence of blebbistatin, a myosin II inhibitor, showed that bleb formation correlated with migratory capacity, suggesting a functional role for blebs in migration. Bleb formation might be a useful mechanism to improve cell migration in cellular therapy protocols.engAtribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/journal article10.1371/journal.pone.0150004open accessBiología molecularCitologíaCélulaBiología celular