α-Tocopheryl Succinate-Based Polymeric Nanoparticles for the Treatment of Head and Neck Squamous Cell Carcinoma

Sánchez Rodríguez, CarolinaPalao Suay, RaquelRodrigáñez Riesco, LauraAguilar de Armas, María RosaMartín Saldaña, SergioSan Román, JulioSanz Fernández, Ricardo2018-10-292018-10-292018Sánchez-Rodríguez, C., Palao-Suay, R., Rodrigáñez, L., Aguilar, M., Martín-Saldaña, S., San Román, J., & Sanz-Fernández, R. (2018). α-Tocopheryl Succinate-Based Polymeric Nanoparticles for the Treatment of Head and Neck Squamous Cell Carcinoma. Biomolecules, 8(3), 97. http://dx.doi.org/10.3390/biom80300972218-273Xhttp://hdl.handle.net/11268/7525The aim of this work is to study, in an in vitro head and neck squamous cell carcinomas model the anti-angiogenic and anti-migratory properties of self-assembled polymeric nanoparticles (NPs) with demonstrated selective anticancer activity. The NPs are based on -tocopheryl succinate ( -TOS) encapsulated in the hydrophobic core of the NPs. We analyzed the effect of the newly synthetized -TOS-loaded NPs in proliferating endothelial cells and hypopharynx carcinoma squamous cells and measured markers of angiogenesis, apoptosis and reactive oxygen species (ROS). -TOS-loaded NPs suppressed angiogenesis by inducing accumulation of ROS and inducing apoptosis of proliferating endothelial cells. These NPs also decrease the number and quality of capillary-like tubes in an in vitro three-dimensional (3D) experiment, decrease the production of the pro-angiogenic vascular endothelial growth factor and down-regulate the expression of its receptor. The anti-migratory efficacy of -TOS is corroborated in hypopharynx carcinoma cells by decreasing the secretion of matrix metalloproteases 2 and 9 (MMP-2 and MMP-9) and inhibiting cell migration. These results confirm that -TOS-based NPs not only present anticancer properties, but also antiangiogenic properties, therefore making them promising candidates for multi-active combinatorial anticancer therapy.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/α-Tocopheryl Succinate-Based Polymeric Nanoparticles for the Treatment of Head and Neck Squamous Cell Carcinomajournal article10.3390/biom8030097open accessNanotecnologíaPolímerosNanotecnologíaPolímero