Romero Fernández, MaríaMoreno Pérez, SoniaMartins de Oliveira, SandroSantamaría, Ramón I.Guisan, José M.Rocha Martín, Javier2019-07-092019-07-092018Romero-Fernández, M., Moreno-Perez, S., Martins de Oliveira, S., Santamaría, R. I., Guisan, J. M., & Rocha-Martin, J. (2018). Preparation of a robust immobilized biocatalyst of β-1,4-endoxylanase by surface coating with polymers for production of xylooligosaccharides from different xylan sources. New Biotechnology, 44, 50–58. https://doi.org/https://doi.org/10.1016/j.nbt.2018.04.0071359-51131871-6784http://hdl.handle.net/11268/8097Xylooligosaccharides display interesting prebiotic effects on human health. The endoxylanase Xys1Δ, from Streptomyces halstedii JM8, was immobilized and stabilized on glyoxyl-agarose beads by multipoint covalent attachment using a novel strategy based on surface coating with a multilayer of polymers. The optimal modification consisted of surface coating with a bilayer formed by a layer of derived dextran polymers and a layer of polyethylenimine. The optimized biocatalyst was 550-fold more stable than one-point covalent immobilized Xys1Δ (at 70 °C, pH 7). This biocatalyst was tested for the production of xylooligosaccharides from soluble xylans from various sources. Hydrolysis of beechwood, wheat straw and corncob xylans was 93% in 4 h, 44% in 5 h and 100% in 1 h, respectively. Maximum values of xylooligosaccharides were found for beechwood at 20.6 mg/mL, wheat at 12.5 mg/mL and corncob at 30.4 mg/mL. The optimized biocatalyst was reused for 15 reaction cycles without affecting its catalytic activityengjournal article10.1016/j.nbt.2018.04.007restricted accessMicrobiologíaEnzimasMicrobiología