TY - JOUR 
A1 - Santos Moriano, Paloma Carmen
AU - Kidibule, Peter E.
AU - Alleyne, Elena
AU - Ballesteros, Antonio O.
AU - Heras, Ángeles
AU - Fernández Lobato, María
AU - Plou, Francisco J.
T1 - Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis
Y1 - 2018
SN - 1359-5113

UR - http://hdl.handle.net/11268/8118
AB - Starting from a commercial preparation of Bacillus thuringiensis var. aizawai, widely employed as biopesticide in agriculture, we obtained a chitosanolytic activity by washing the cells with concentrated sodium acetate. The optimum pH and temperature values for this enzyme were 6.0 and 60 °C, respectively. The enzymatic extract efficiently hydrolyzed various chitosans with different deacetylation degrees (DD) into small oligosaccharides. Combining mass spectrometry (ESI-Q-TOF) and high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), we observed the formation of fully deacetylated chitooligosaccharides [fdCOS, (GlcN)2-5] and, to a lesser extent, partially acetylated chitooligosaccharides [paCOS, GlcNAc-(GlcN)1-3]. A 10 g/L solution of chitosan with 600–800 kDa and DD ≥ 90% was fully converted into oligosaccharides in 55 h. Most of the products were fdCOS: 1.6 g/L of chitobiose, 1.7 g/L of chitotriose, 5 g/L of chitotetraose and 1.4 g/L of chitopentaose.
KW - Quitones
KW - Oligosacáridos
KW - Bioquímica
KW - Bioquímica
LA - eng
ER -