TY - JOUR 
A1 - Sánchez Rodríguez, Carolina
AU - Peraza Cruces, Keyliz Rossmery
AU - Rodrigáñez Riesco, Laura
AU - García Vela, José Antonio
AU - Sanz Fernández, Ricardo
T1 - Immunomodulatory effect of Polypodium leucotomos (Anapsos) in child palatine tonsil model
Y1 - 2018
SN - 0165-5876

UR - http://hdl.handle.net/11268/7536
AB - Background: Recurrent tonsillitis might reduce the immunological capability of fighting against the infection of
tonsil tissue. Polypodium leucotomos (Anapsos) immunomodulating effect has been subject of research in the last
years. The aim of this research is to test the in vitro immunomodulating capacity of Anapsos in a child palatine
tonsil explants model.
Methods: Palatine tonsils explants of children undergoing amigdalectomy were stimulated with mononuclear
cells obtained from their own blood by density gradient centrifugation. Some were then treated with Anapsos
while others rest untreated. Cytokines were measured by ELISA, immune cells activation was measured by flow
cytometry and activation of immunoglobulins was appreciated by indirect immunofluorescence in tonsils tissue.
Results: Anapsos activates Natural Killers cells. It increases IL-2 and IFN-γ levels by the activation of Th2 lymphocytes,
and IL-10, by the Th1 lymphocytes. Anapsos also increases immunoglobulins IgM, IgD and IgG4 by Blymphocyte
activation in tonsils tissue.
Conclusion: Anapsos has an immunomodulating effect, both in humoral and cellular responses, which might
benefit children suffering of recurrent tonsillitis as it could enhance their immune system. This effect might
reduce the number of episodes suffered and therefore the number of children undergoing surgery.
KW - Enfermedades
KW - Pediatria
KW - Enfermedad
KW - Pediatría
LA - eng
ER -