TY - JOUR A1 - Martínez Caro, Leticia AU - Lorente Balanza, José Ángel AU - Marín Corral, J. AU - Sánchez Rodríguez, Carolina AU - Sánchez Ferrer, A. AU - Nin, Nicolás AU - Ferruelo, Antonio AU - Paula, Marta de AU - Fernández Segoviano, P. AU - Barreiro, Esther AU - Esteban, Andrés T1 - Role of free radicals in vascular dysfunction induced by high tidal volume ventilation Y1 - 2009 SN - 00903493 UR - http://hdl.handle.net/11268/4201 AB - The objective is to demonstrate that increased formation of reactive oxygen (ROS) and nitrogen species (RNS) is involved in VILI-induced vascular dysfunction. Male Sprague-Dawley anesthetized rats were ventilated for 60 min using low V(T) ventilation [V(T) 9 ml/kg, positive end-expiratory pressure (PEEP) 5 cmH(2)O, n = 18], and high V(T) ventilation (V(T) 35 ml/kg, zero PEEP, n = 18). Arterial pressure and respiratory system mechanics were monitored. Blood samples for the determination of arterial blood gases and lactate concentration were drawn. Vascular rings from the thoracic aortae were mounted in organ baths for isometric tension recording. We studied endothelium-dependent relaxation in norepinephrine-precontracted rings (acetylcholine, 10 nM-10 microM) and contraction induced by norepinephrine (1 nM-10 microM) in resting vessels. Vascular rings were preincubated for 30 min with Zn-Mn-SOD (100 u/ml) or tempol (10(-4) M) (extracellular and intracellular superoxide scavengers, respectively) or MnTMPyP (10(-5) M) (a superoxide and peroxynitrite scavenger). KW - Cardiología KW - Disfunción vascular KW - Aparato circulatorio KW - Sistema cardiovascular LA - spa ER -