TY - JOUR 
A1 - Montero Magalló, Paula
AU - Roger Laparra, Inés
AU - Estornut Navarro, Cristina
AU - Milara, Javier
AU - Cortijo, Julio
T1 - Influence of dose and exposition time in the effectiveness of N-Acetyl-L-cysteine treatment in A549 human epithelial cells
Y1 - 2023
SN - 2405-8440
UR - http://hdl.handle.net/11268/11989
AB - N-Acetyl-l-cysteine (NAC) acts as a precursor of the tripeptide glutathione (GSH), one of the principal cell mechanisms for reactive oxygen species (ROS) detoxification. Chronic obstructive pulmonary disease (COPD) is associated with enhanced inflammatory response and oxidative stress and NAC has been used to suppress various pathogenic processes in this disease. Studies show that the effects of NAC are dose-dependent, and it appears that the efficient doses in vitro are usually higher than the achieved in vivo plasma concentrations. However, to date, the inconsistencies between the in vitro NAC antioxidant and anti-inflammatory in vitro effects, by reproducing the in vivo NAC plasma concentrations as well as high NAC concentrations. To do so, A549 were transfected with polyinosinic-polycytidylic acid (Poly (I:C)) and treated with NAC at different treatment periods. Oxidative stress, release of proinflammatory mediators and NFkB activation were analyzed. Results suggest that NAC at low doses in chronic administration has sustained antioxidant and anti-inflammatory effects, while acute treatment with high dose NAC exerts a strong antioxidant and anti-inflammatory response.
KW - Acetilcisteína
KW - Estrés oxidativo
KW - Enfermedad pulmonar obstructiva crónica
KW - Aparato respiratorio
KW - Enfermedad
KW - Medicamento
LA - eng
ER -