TY - JOUR 
A1 - Fernández Novo, Aitor
AU - Santos López, Sergio
AU - Barrajón Masa, Clara
AU - Mozas, Patricia
AU - Mercado, Eduardo de
AU - Cáceres, Elisa
AU - Garrafa, Aizic
AU - González Martín, Juan Vicente
AU - Pérez Villalobos, Natividad
AU - Oliet, Agustín
AU - Astiz Blanco, Susana
AU - Pérez Garnelo, Sonia S.
T1 - Effect of Extender, Storage Time and Temperature on Kinetic Parameters (CASA) on Bull Semen Samples
Y1 - 2021
SN - 2079-7737
UR - http://hdl.handle.net/11268/10411
AB - CASA kinetic parameters are often evaluated in a diagnostic centre. How storage conditions affect ejaculates up to evaluation is unclear. We assessed, in 25 commercial bulls electroejaculated in the field, the impact of time until evaluation (0–2 h, 4–6 h, and 24 h post-ejaculation), holding temperature (5 °C vs. room temperature), and extender (AndroMed®, BIOXcell® or INRA96®) on CASA kinetic parameters. Total and progressive motility, VCL, VAP, VCL, ALH, BCF, STR, LIN, and WOB were assessed. CASA kinetic parameters were preserved for up to 4–6 h post-ejaculation, except for AndroMed®. Regardless of extender or temperature, motility decreased from 4–6 h up to 24 h, with the best values obtained with BIOXcell® at 5 °C. Our results suggest that BIOXcell® can preserve sperm motility for up to 6 h, either at 5 °C or room temperature, and also INRA96® at room temperature, with motility assessments and the percentage of the most rapid sperms being the lowest with INRA96® at 5 °C. The kinetic parameters decreased when analyses were performed at 24 h. Therefore, we suggest evaluating seminal quality as soon as possible, before 6 h after collection. These results help to fix adequate protocols for the short-term storage and shipment of bovine semen collected under field conditions.
KW - Fertilización
KW - Semen
KW - Medicina veterinaria
KW - Biología
KW - Fecundidad
KW - Ganado vacuno
LA - eng
ER -