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dc.contributor.author | Santos Moriano, Paloma Carmen | |
dc.contributor.author | Kidibule, Peter E. | |
dc.contributor.author | Alleyne, Elena | |
dc.contributor.author | Ballesteros, Antonio O. | |
dc.contributor.author | Heras, Ángeles | |
dc.contributor.author | Fernández Lobato, María | |
dc.contributor.author | Plou, Francisco J. | |
dc.date.accessioned | 2019-07-10T17:23:31Z | |
dc.date.available | 2019-07-10T17:23:31Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Santos-Moriano, P., Kidibule, P. E., Alleyne, E., Ballesteros, A. O., Heras, A., Fernandez-Lobato, M., & Plou, F. J. (2018). Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis. Process Biochemistry, 73, 102–108. https://doi.org/10.1016/j.procbio.2018.07.017 | spa |
dc.identifier.issn | 1359-5113 | |
dc.identifier.issn | 1873-3298 | |
dc.identifier.uri | http://hdl.handle.net/11268/8118 | |
dc.description.abstract | Starting from a commercial preparation of Bacillus thuringiensis var. aizawai, widely employed as biopesticide in agriculture, we obtained a chitosanolytic activity by washing the cells with concentrated sodium acetate. The optimum pH and temperature values for this enzyme were 6.0 and 60 °C, respectively. The enzymatic extract efficiently hydrolyzed various chitosans with different deacetylation degrees (DD) into small oligosaccharides. Combining mass spectrometry (ESI-Q-TOF) and high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), we observed the formation of fully deacetylated chitooligosaccharides [fdCOS, (GlcN)2-5] and, to a lesser extent, partially acetylated chitooligosaccharides [paCOS, GlcNAc-(GlcN)1-3]. A 10 g/L solution of chitosan with 600–800 kDa and DD ≥ 90% was fully converted into oligosaccharides in 55 h. Most of the products were fdCOS: 1.6 g/L of chitobiose, 1.7 g/L of chitotriose, 5 g/L of chitotetraose and 1.4 g/L of chitopentaose. | spa |
dc.description.sponsorship | Sin financiación | spa |
dc.language.iso | eng | spa |
dc.title | Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis | spa |
dc.type | article | spa |
dc.description.impact | 2.883 JCR (2018) Q2, 148/299 Biochemistry & Molecular Biology, 59/162 Biotechnology & Applied Microbiology, 47/138 Engineering, Chemical | spa |
dc.description.impact | 0.754 SJR (2018) Q2, 213/462 Biochemistry, 57/188 Bioengineering, 37/114 Applied Microbiology and Biotechnology | spa |
dc.description.impact | No data IDR 2018 | spa |
dc.identifier.doi | 10.1016/j.procbio.2018.07.017 | |
dc.rights.accessRights | closedAccess | spa |
dc.subject.uem | Quitones | spa |
dc.subject.uem | Oligosacáridos | spa |
dc.subject.uem | Bioquímica | spa |
dc.subject.unesco | Bioquímica | spa |
dc.description.filiation | UEM | spa |
dc.peerreviewed | Si | spa |
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