Resumen:
The present study examined the mRNA expression
levels of different isoforms of the plasma membrane calcium
ATPase 2 (PMCA2) gene generated by alternative splicing
at the first intracellular loop (site A) and C‑terminal region
(site C) in 85 human breast cancer tumor and 69 adjacent
non-tumor tissues. Associations were identified between
the expression of PMCA2 splice isoforms and the following
clinical variables: Estrogen receptor (ER), progesterone
receptor (PR) and human epidermal growth factor receptor 2
(HER2) status, tumor size, staging and histological classification,
and lymph node status. Transcripts including splice
site A or splice site C were amplified by reverse transcription-quantitative
polymerase chain reaction using PMCA2
isoform‑specific primers. Tumor and adjacent tissues were
determined to express the different PMCA2 splice isoforms
2w, 2x and 2z (site A), and 2b (site C). The mRNA levels
for these variants indicated high biological variability, but
increased expression was observed in breast tumor tissues,
compared with in adjacent tissues. Significantly increased
PMCA2x/b expression levels were detected in breast tumor
tissues histologically classified as ...