dc.contributor.author |
Azami-Conesa, Iris |
|
dc.contributor.author |
Matas Mendez, Pablo |
|
dc.contributor.author |
Perez-Moreno, Paula |
|
dc.contributor.author |
Carrion, Javier |
|
dc.contributor.author |
Alunda, Jose Maria |
|
dc.contributor.author |
Mateo Barrientos, Marta |
|
dc.contributor.author |
Gómez Muñoz, Teresa |
|
dc.date.accessioned |
2024-09-15T07:45:29Z |
|
dc.date.available |
2024-09-15T07:45:29Z |
|
dc.date.issued |
2024 |
|
dc.identifier.citation |
Azami-Conesa, I., Matas Méndez, P., Pérez-Moreno, P., Carrión, J., Alunda, J. M., Mateo Barrientos, M., & Gómez-Muñoz, M. T. (2024). Wildlife as a sentinel for pathogen introduction in nonendemic areas: first detection of Leishmania tropica in wildlife in Spain. Transboundary and Emerging Diseases, 2024(1), 10. https://doi.org/10.1155/2024/8259712 |
spa |
dc.identifier.issn |
1865-1674 |
|
dc.identifier.issn |
1865-1682 |
|
dc.identifier.uri |
http://hdl.handle.net/11268/13039 |
|
dc.description.abstract |
Leishmaniasis is a chronic global arthropod-borne zoonotic disease produced by several species of Leishmania with cutaneous,
mucocutaneous, and visceral clinical manifestations. In Spain, only Leishmania infantum has been reported so far, although other
species of Leishmania, such as L. tropica and L. major, are present in surrounding countries. The aim of this work is to analyze the
occurrence of Leishmania spp. infection in European wildcats (Felis silvestris) as sentinels, including their genotypic characterization.
Necropsies of 18 road-killed wildcats were conducted. Samples of ear skin and spleen were taken for DNA isolation and PCR
of the highly sensitive SSU-rDNA target. Subsequent PCR tests were performed using more specific targets for the determination of
Leishmania species: hsp70 and ITS1. Positive samples were sequenced, and phylogenetic trees were constructed. Seven wildcats
were found positive for Leishmania spp. Based on the hsp70 and ITS1 sequences, an animal was found to be infected only with
L. tropica in ear skin samples, while two cats were found to be infected with L. infantum in both the ear skin and the spleen. In one
animal, a clear sequence of L. infantum ITS1 and a sequence of L. tropica hsp70 were obtained from the ear skin. Since hsp70 and
ITS1 sequencing was not possible in three cats, the species of Leishmania infecting them was not determined. This is the first report
of autochthonous infection with L. tropica in the Iberian Peninsula. Health care professionals, including physicians, dermatologists,
and veterinarians, must be aware of this for a correct diagnosis, treatment, and management of possible coinfections. |
spa |
dc.description.sponsorship |
The research was partially funded by the ICPVet research group of UCM (grant GRFN17/21). |
spa |
dc.language.iso |
eng |
spa |
dc.rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
|
dc.rights.uri |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
dc.title |
Wildlife as a Sentinel for Pathogen Introduction in Nonendemic Areas: First Detection of Leishmania tropica in Wildlife in Spain |
spa |
dc.type |
article |
spa |
dc.description.impact |
3.5 Q2 JCR 2023; 0.921 Q1 SJR 2024; No data IDR 2022 |
spa |
dc.description.impact |
|
|
dc.description.impact |
|
|
dc.identifier.doi |
10.1155/2024/8259712 |
|
dc.rights.accessRights |
openAccess |
spa |
dc.subject.unesco |
Parasitología |
spa |
dc.subject.unesco |
Fauna |
spa |
dc.subject.unesco |
España |
spa |
dc.description.filiation |
UEM |
spa |
dc.relation.publisherversion |
https://doi.org/10.1155/2024/8259712 |
spa |
dc.peerreviewed |
Si |
spa |