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dc.contributor.author | Molina Espeja, Patricia | |
dc.contributor.author | Santos Moriano, Paloma Carmen | |
dc.contributor.author | García Ruiz, Eva | |
dc.contributor.author | Ballesteros, Antonio O. | |
dc.contributor.author | Plou, Francisco J. | |
dc.contributor.author | Alcalde, Miguel | |
dc.date.accessioned | 2022-02-21T15:36:12Z | |
dc.date.available | 2022-02-21T15:36:12Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Molina-Espeja, P., Santos-Moriano, P., García-Ruiz, E., Ballesteros, A., Plou, F. J., & Alcalde, M. (2019). Structure-guided immobilization of an evolved unspecific peroxygenase. International Journal of Molecular Sciences, 20(7), 1627. https://doi.org/10.3390/ijms20071627 | spa |
dc.identifier.issn | 1422-0067 | |
dc.identifier.uri | http://hdl.handle.net/11268/10787 | |
dc.description.abstract | Unspecific peroxygenases (UPOs) are highly promiscuous biocatalyst with self-sufficient mono(per)oxygenase activity. A laboratory-evolved UPO secreted by yeast was covalently immobilized in activated carriers through one-point attachment. In order to maintain the desired orientation without compromising the enzyme’s activity, the S221C mutation was introduced at the surface of the enzyme, enabling a single disulfide bridge to be established between the support and the protein. Fluorescence confocal microscopy demonstrated the homogeneous distribution of the enzyme, regardless of the chemical nature of the carrier. This immobilized biocatalyst was characterized biochemically opening an exciting avenue for research into applied synthetic chemistry. | spa |
dc.description.sponsorship | Repsol S.A and the EU project H2020-BBI-PPP2015-2-720297-ENZOX2. | spa |
dc.language.iso | eng | spa |
dc.rights | Attribution 4.0 International (CC BY 4.0) | * |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.other | Técnicas de química sintética | spa |
dc.title | Structure-Guided Immobilization of an Evolved Unspecific Peroxygenase | spa |
dc.type | article | spa |
dc.description.impact | 4.556 JCR (2019) Q1, 74/297 Biochemistry & Molecular Biology | spa |
dc.description.impact | 1.317 SJR (2019) Q1, 93/2331 Computer Science Applications | spa |
dc.description.impact | No data IDR 2019 | spa |
dc.identifier.doi | 10.3390/ijms20071627 | |
dc.rights.accessRights | openAccess | spa |
dc.subject.unesco | Investigación empírica | spa |
dc.subject.unesco | Enzima | spa |
dc.subject.unesco | Química | spa |
dc.description.filiation | UEM | spa |
dc.peerreviewed | Si | spa |